کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5522276 1545904 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Applications of copolymer for rapid identification of bacteria in blood culture broths using matrix-assisted laser desorption ionization time-of-flight mass spectrometry
ترجمه فارسی عنوان
کاربرد کوپلیمر برای شناسایی سریع باکتری ها در محفظه های کشت خون با استفاده از اسپکترومتر جرمی یونیزاسیون لیزر جذب ماتریکس
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
چکیده انگلیسی


- We developed our protocol using a copolymer of polyallylamine and polystyrene.
- Our pretreatment method identifies bacteria in blood culture broth by MALDI-TOF MS.
- Our protocol is a rapid method of collecting bacteria within approximately 15 min.
- Significantly less hemoglobin interference was seen than in a conventional method.

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) can be used to identify pathogens in blood culture samples. However, sample pretreatment is needed for direct identification of microbes in blood culture bottles. Conventional protocols are complex and time-consuming. Therefore, in this study, we developed a method for collecting bacteria using polyallylamine-polystyrene copolymer for application in wastewater treatment technology. Using representative bacterial species Escherichia coli and Staphylococcus capitis, we found that polyallylamine-polystyrene can form visible aggregates with bacteria, which can be identified using MALDI-TOF MS. The processing time of our protocol was as short as 15 min. Hemoglobin interference in MALDI spectra analysis was significantly decreased in our method compared with the conventional method. In a preliminary experiment, we evaluated the use of our protocol to identify clinical isolates from blood culture bottles. MALDI-TOF MS-based identification of 17 strains from five bacterial species (E. coli, Klebsiella pneumoniae, Enterococcus faecalis, S. aureus, and S. capitis) collected by our protocol was satisfactory. Prospective large-scale studies are needed to further evaluate the clinical application of this novel and simple method of collecting bacteria in blood culture bottles.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 139, August 2017, Pages 54-60
نویسندگان
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