کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5526909 1401555 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Phosphoinositide phosphatase Sac3 regulates the cell surface expression of scavenger receptor A and formation of lipid droplets in macrophages
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Phosphoinositide phosphatase Sac3 regulates the cell surface expression of scavenger receptor A and formation of lipid droplets in macrophages
چکیده انگلیسی


- Sac3 knockdown decreased the foam cell formation in RAW264.7 macrophages.
- Sac3 knockdown decreased the level of the cell surface SR-A.
- ArPIKfyve knockdown decreased Sac3, cell surface SR-A, and foam cell formation.
- The knockdown of PIKfyve had no effect on the cell surface SR-A.
- ArPIKfyve-Sac3 complex regulates SR-A protein levels independently of PIKfyve.

The findings of this study suggest that the phosphoinositide phosphatase Sac3 maintains the protein level of scavenger receptor A (SR-A) and regulates foam cell formation. RAW264.7 macrophages were transfected with short hairpin RNAs that target Sac3. The knockdown decreased the level of the cell surface SR-A and suppressed the acetylated low density lipoprotein-induced foam cell formation. The associated regulator of PIKfyve (ArPIKfyve) is a scaffold protein that protects Sac3 from proteasome-dependent degradation. The knockdown of ArPIKfyve decreased Sac3, cell surface SR-A, and foam cell formation. The knockdown of PIKfyve had no effect on SR-A protein levels. These results suggest that the ArPIKfyve-Sac3 complex regulates SR-A protein levels independently of its effect on PIKfyve activity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 357, Issue 2, 15 August 2017, Pages 252-259
نویسندگان
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