کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5527006 1401560 2017 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The atypical Rho GTPase RhoD is a regulator of actin cytoskeleton dynamics and directed cell migration
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
The atypical Rho GTPase RhoD is a regulator of actin cytoskeleton dynamics and directed cell migration
چکیده انگلیسی


- Increased RhoD expression leads to loss of actin structures, e.g. stress fibers and gives rise to decreased actin dynamics.
- RhoD knockdown induces various actin-containing structures such as edge ruffles, stress fibers and cortical actin, in a cell-type specific manner.
- RhoD induces specific actin rearrangements depending on its subcellular localization.
- RhoD knockdown has effects on cellular processes, such as directed cell migration and proliferation.

RhoD belongs to the Rho GTPases, a protein family responsible for the regulation and organization of the actin cytoskeleton, and, consequently, many cellular processes like cell migration, cell division and vesicle trafficking. Here, we demonstrate that the actin cytoskeleton is dynamically regulated by increased or decreased protein levels of RhoD. Ectopic expression of RhoD has previously been shown to give an intertwined weave of actin filaments. We show that this RhoD-dependent effect is detected in several cell types and results in a less dynamic actin filament system. In contrast, RhoD depletion leads to increased actin filament-containing structures, such as cortical actin, stress fibers and edge ruffles. Moreover, vital cellular functions such as cell migration and proliferation are defective when RhoD is silenced. Taken together, we present data suggesting that RhoD is an important component in the control of actin dynamics and directed cell migration.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 352, Issue 2, 15 March 2017, Pages 255-264
نویسندگان
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