کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5527095 1401563 2016 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research ArticleQuantitative evaluation of single cell spread on collagen matrices
ترجمه فارسی عنوان
مقاله پژوهشی مقیاس سنجش میزان تک سلولی بر روی ماتریس کلاژن
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
چکیده انگلیسی


- Automated scoring of cell morphology is a valuable (drug) screening tool.
- MAS allows quantitative evaluation of single cell spread on collagen matrices.
- TGFα is a stimulator of morphological changes correlated with migration.

Cells change their morphology as a response to environmental cues. The quantitative evaluation of single cell spread on extracellular matrices, such as type I collagen, is a key tool in cancer research. Inherent to the manual scoring of cellular spread is inter-observer but also intra-observer variation. To overcome these problems, we have developed the Morphology Analysis Software (MAS). MAS scores phase-contrast images of cells on native type I collagen gels and identifies whether a cell has a spread or round morphology using a combination of four unique parameters: the presence of a cellular extension, the cell area, the cell eccentricity and cell circularity. The MAS software scores are equivalent to the average score of five independent observers but MAS is faster, more objective and standardized. A functional screening assay using six cytokines identified TGFα as a stimulator of HCT8/E11 and SK-BR-3 single cell spreading on top of type I collagen gels. This change in morphology correlates with increased migration potential as evidenced by xCELLigence migration assays and are counteracted by EGFR signaling pathway inhibitors. This underscores the use of morphology classification on a population of unlabeled cells as read-out of an important cancer cell property and the potential for the MAS software in drug screening strategies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 349, Issue 1, 15 November 2016, Pages 168-178
نویسندگان
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