کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5527300 1401576 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Epithelial Membrane Protein 2 and β1 integrin signaling regulate APC-mediated processes
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Epithelial Membrane Protein 2 and β1 integrin signaling regulate APC-mediated processes
چکیده انگلیسی


- β1 integrin is upregulated in APC knockdown MDCK cells.
- APC loss increases cell motility during wound-healing assays.
- EMP2 regulates APC-mediated cyst size and apical polarity, but has no impact on migration.
- β1 integrin signaling mediates cyst size and migration due to APC loss, with no effect on polarity.

Adenomatous Polyposis Coli (APC) plays a critical role in cell motility, maintenance of apical-basal polarity, and epithelial morphogenesis. We previously demonstrated that APC loss in Madin Darby Canine Kidney (MDCK) cells increases cyst size and inverts polarity independent of Wnt signaling, and upregulates the tetraspan protein, Epithelial Membrane Protein 2 (EMP2). Herein, we show that APC loss increases β1 integrin expression and migration of MDCK cells. Through 3D in vitro model systems and 2D migration analysis, we have depicted the molecular mechanism(s) by which APC influences polarity and cell motility. EMP2 knockdown in APC shRNA cells revealed that APC regulates apical-basal polarity and cyst size through EMP2. Chemical inhibition of β1 integrin and its signaling components, FAK and Src, indicated that APC controls cyst size and migration, but not polarity, through β1 integrin and its downstream targets. Combined, the current studies have identified two distinct and novel mechanisms required for APC to regulate polarity, cyst size, and cell migration independent of Wnt signaling.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 350, Issue 1, 1 January 2017, Pages 190-198
نویسندگان
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