کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5527321 1401577 2016 16 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research ArticleThe gene expression profile of non-cultured, highly purified human adipose tissue pericytes: Transcriptomic evidence that pericytes are stem cells in human adipose tissue
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Research ArticleThe gene expression profile of non-cultured, highly purified human adipose tissue pericytes: Transcriptomic evidence that pericytes are stem cells in human adipose tissue
چکیده انگلیسی


- Non-cultured adipose tissue-derived human pericytes (ncATPCs) exhibit a distinctive gene expression signature.
- ncATPCs express key adipose tissue stem cell genes previously described in vivo in mice.
- ncATPCs express message for anti-proliferative and antiangiogenic molecules.
- Most ncATPC-specific transcripts are absent in culture-expanded pericytes or ATMSCs
- Gene expression changes ncATPCs undergo as they acquire a cultured ATMSC phenotype are pointed out.

Pericytes (PCs) are a subset of perivascular cells that can give rise to mesenchymal stromal cells (MSCs) when culture-expanded, and are postulated to give rise to MSC-like cells during tissue repair in vivo. PCs have been suggested to behave as stem cells (SCs) in situ in animal models, although evidence for this role in humans is lacking. Here, we analyzed the transcriptomes of highly purified, non-cultured adipose tissue (AT)-derived PCs (ATPCs) to detect gene expression changes that occur as they acquire MSC characteristics in vitro, and evaluated the hypothesis that human ATPCs exhibit a gene expression profile compatible with an AT SC phenotype. The results showed ATPCs are non-proliferative and express genes characteristic not only of PCs, but also of AT stem/progenitor cells. Additional analyses defined a gene expression signature for ATPCs, and revealed putative novel ATPC markers. Almost all AT stem/progenitor cell genes differentially expressed by ATPCs were not expressed by ATMSCs or culture-expanded ATPCs. Genes expressed by ATMSCs but not by ATPCs were also identified. These findings strengthen the hypothesis that PCs are SCs in vascularized tissues, highlight gene expression changes they undergo as they assume an MSC phenotype, and provide new insights into PC biology.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 349, Issue 2, 10 December 2016, Pages 239-254
نویسندگان
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