کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5530683 | 1549381 | 2017 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Research paperSTRAP positively regulates TLR3-triggered signaling pathway Research paperSTRAP positively regulates TLR3-triggered signaling pathway](/preview/png/5530683.png)
- STRAP functions as a scaffold protein in TLR3-triggered signaling pathway.
- STRAP specifically binds to TBK1 and IRF3.
- The C-terminus of STRAP is required for its functional activity in TLR3-mediated IFN-β production.
Toll-like receptor (TLR) signaling drives the innate immune response by activating nuclear factor-κB (NF-κB) and interferon regulatory factor (IRF). We have previously shown that STRAP interacts with TAK1 and IKKα along with NF-κB subunit p65, leading to the activation of pro-inflammatory cytokines. However, the roles of STRAP in TRIF/TBK1-mediated TLR3 activation and the subsequent type I interferon (IFN) production are not fully elucidated. Here, we demonstrate that STRAP acts as a scaffold protein in TLR3-triggered signaling. STRAP strongly interacts with TBK1 and IRF3, which enhances IFN-β production. As a consequence, STRAP knockdown reduces the level of both pro-inflammatory cytokine and IFN in TLR3 agonist-stimulated macrophages, whereas its overexpression significantly enhances production of these cytokines. Furthermore, the C-terminus of STRAP is essential for its functional activity in TLR3-mediated IL-6 and IFN-β production. These data suggest that STRAP is a positive regulator of the TLR3-meditated NF-κB and IRF signaling pathway.
Journal: Cellular Immunology - Volume 318, August 2017, Pages 55-60