کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5536794 | 1402306 | 2016 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Protection against henipaviruses in swine requires both, cell-mediated and humoral immune response
ترجمه فارسی عنوان
حفاظت در برابر گرمی ویروس ها در خوک به هر دو، پاسخ ایمنی سلولی و هومورال نیاز دارد
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
چکیده انگلیسی
Hendra virus (HeV) and Nipah virus (NiV) are members of the genus Henipavirus, within the family Paramyxoviridae. Nipah virus has caused outbreaks of human disease in Bangladesh, Malaysia, Singapore, India and Philippines, in addition to a large outbreak in swine in Malaysia in 1998/1999. Recently, NiV was suspected to be a causative agent of an outbreak in horses in 2014 in the Philippines, while HeV has caused multiple human and equine outbreaks in Australia since 1994. A swine vaccine able to prevent shedding of infectious virus is of veterinary and human health importance, and correlates of protection against henipavirus infection in swine need to be better understood. In the present study, three groups of animals were employed. Pigs vaccinated with adjuvanted recombinant soluble HeV G protein (sGHEV) and challenged with HeV, developed antibody levels considered to be protective prior to the challenge (titers of 320). However, activation of the cell-mediated immune response was not detected, and the animals were only partially protected against challenge with 5Â ÃÂ 105 PFU of HeV per animal. In the second group, cross-neutralizing antibody levels against NiV in the sGHEV vaccinated animals did not reach protective levels, and with no activation of cellular immune memory, these animals were not protected against NiV. Only pigs orally infected with 5Â ÃÂ 104 PFU of NiV per animal were protected against nasal challenge with 5Â ÃÂ 105 PFU of NiV per animal. This group of pigs developed protective antibody levels, as well as cell-mediated immune memory. Peripheral blood mononuclear cells restimulated with UV-inactivated NiV upregulated IFN-gamma, IL-10 and the CD25 activation marker on CD4+CD8+ T memory helper cells and to lesser extent on CD4âCD8+ T cells. In conclusion, both humoral and cellular immune responses were required for protection of swine against henipaviruses.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 34, Issue 40, 14 September 2016, Pages 4777-4786
Journal: Vaccine - Volume 34, Issue 40, 14 September 2016, Pages 4777-4786
نویسندگان
Brad S. Pickering, John M. Hardham, Greg Smith, Eva T. Weingartl, Paul J. Dominowski, Dennis L. Foss, Duncan Mwangi, Christopher C. Broder, James A. Roth, Hana M. Weingartl,