کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5539430 1402422 2017 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Efficacy of α-enolase-based DNA vaccine against pathogenic Streptococcus iniae in Nile tilapia (Oreochromis niloticus)
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
Efficacy of α-enolase-based DNA vaccine against pathogenic Streptococcus iniae in Nile tilapia (Oreochromis niloticus)
چکیده انگلیسی
Streptococcus iniae (SI) is an important pathogenic bacterium causing severe mortality in farmed fish worldwide. In the current study, the α-enolase-based DNA vaccine was constructed and examined for its effectiveness against SI infection in Nile tilapia (Oreochromis niloticus). The juvenile tilapia were immunized intramuscularly with DNA vaccine, pEno, and kept for 30 days prior to the intraperitoneal challenge with 2.7 × 107 CFU of pathogenic SI. At two weeks post challenge, the pEno group yielded the highest survival rate at 72.5%, whereas mock vaccination and negative control groups gained only 40 and 25%, respectively. The protection of vaccine tended to be related to the expression of immune-relevant genes (IL-1β, TNF-α, COX-2, IL-12β and IL-13Rα1) at 7 day post-vaccination (dpv) and the anti-SI serum antibody level at 30 dpv (before in vivo challenge). This study indicated that pEno was able to elicit immune responses and conferred protection against streptococcosis associated with SI infection in Nile tilapia.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquaculture - Volume 468, Part 1, 1 February 2017, Pages 102-106
نویسندگان
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