کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5540030 | 1553562 | 2017 | 44 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular characterization and expression analysis of three subclasses of IgT in rainbow trout (Oncorhynchus mykiss)
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کلمات کلیدی
LLRV domainJ segmentC domainjoining segmentRAGIgHRSSVLRBCRqPCRIGTmAbMonoclonal antibody - آنتی بادی مونوکلونالimmunoglobulin - ایمونوگلوبولینIGL - ایگلrecombination signal sequence - توالی سیگنال بازخوردleucine-rich repeat - تکرار غنی از لوسینConstant domain - دامنه ثابتVariable domain - دامنه متغیرlight chain variable domain - دامنه متغیر زنجیره سبکheavy chain variable domain - دامنه متغیر زنجیره سنگینimmunoglobulin light chain - زنجیره سبک ایمونوگلوبولینImmunoglobulin heavy chain - زنجیره سنگین ایمونوگلوبولینSubclass - زیرمجموعهMass spectrometry - طیف سنجی جرمیRainbow trout - قزل آلای رنگین کمانRace - مسابقهquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیliquid chromatography - کروماتوگرافی مایعB cell receptor - گیرنده سلول BVariable lymphocyte receptor - گیرنده لنفوسیت متغیر
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
As the teleost specific immunoglobulin, IgT plays important roles in systemic and mucosal immunity. In the current study, in rainbow trout, we have cloned the heavy chain (IgÏ) genes of a secretory form of IgT2 as well as the membrane and secretory forms of a third IgT subclass, termed IgT3. Conserved cysteine and tryptophan residues that are crucial for the folding of the immunoglobulin domain as well as hydrophobic and hydrophilic residues within CART motif were identified in all IgT subclasses. Through analysis of the rainbow trout genome assembly, IgÏ3 gene was found localized upstream of IgÏ1 gene, while IgÏ2 gene situated on another scaffold. At the transcriptional level, IgÏ1 was mainly expressed in both systemic and mucosal lymphoid tissues, while IgÏ2 was largely expressed in systemic lymphoid organs. After LPS and poly (I:C) treatment, IgÏ1 and IgÏ2 genes exhibited different expression profiles. Interestingly the transcriptional level of IgÏ3 was negligible, although its protein product could be identified in trout serum. Importantly, a previously reported monoclonal antibody directed against trout IgT1 was able to recognize IgT2 and IgT3. These data demonstrate that there exist three subclasses of IgT in rainbow trout, and that their heavy chain genes display different expression patterns during stimulation. Overall, our data reflect the diversity and complexity of immunoglobulin in trout, thus provide a better understanding of the IgT system in the immune response of teleost fish.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 70, May 2017, Pages 94-105
Journal: Developmental & Comparative Immunology - Volume 70, May 2017, Pages 94-105
نویسندگان
Nu Zhang, Xu-Jie Zhang, Dan-Dan Chen, J. Oriol Sunyer, Yong-An Zhang,