کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5540239 | 1553565 | 2017 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Recombinant IL-4/13A and IL-4/13B induce arginase activity and down-regulate nitric oxide response of primary goldfish (Carassius auratus L.) macrophages
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Recombinant IL-4/13A and IL-4/13B induce arginase activity and down-regulate nitric oxide response of primary goldfish (Carassius auratus L.) macrophages Recombinant IL-4/13A and IL-4/13B induce arginase activity and down-regulate nitric oxide response of primary goldfish (Carassius auratus L.) macrophages](/preview/png/5540239.png)
چکیده انگلیسی
We report on the expression analysis and functional characterization of IL-4/13A and IL-4/13B in goldfish. Quantitative analysis indicated the highest expression in the heart, spleen, brain, and kidney, with comparable expression patterns for both IL-4/13A and IL-4/13B. The mRNA levels of IL-4/13A and IL-4/13B in the immune cells examined were highest in macrophage and monocytes. Assessment of spleen mRNA following infection with Trypanosoma carassii, a prominent protozoan pathogen of fish, revealed decrease in IL-4/13B and arginase expression 14 days post infection, followed by an increase in IL-4/13B and arginase-2 at 28 days post infection. Recombinant forms of IL-4/13A and IL-4/13B induced an increase in arginase activity in macrophages in a dose-dependent manner. Recombinant IL-4/13A and IL-4/13B also induced significant increase in mRNA levels of arginase â2 in macrophages at 6, 12, 18 and 24 h after treatment. Furthermore, treatment with both IL-4/13 recombinants interfered with the IFNγ-induced nitric oxide response of macrophages. Our results suggest a conserved role of IL-4/IL-13 in induction of alternative activation phenotype in teleost macrophages.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 67, February 2017, Pages 377-384
Journal: Developmental & Comparative Immunology - Volume 67, February 2017, Pages 377-384
نویسندگان
Jordan W. Hodgkinson, Chad Fibke, Miodrag Belosevic,