کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5540935 | 1553611 | 2017 | 46 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular characterization and expression analysis of olive flounder (Paralichthys olivaceus) phospholipase C gamma 1 and gamma 2
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
LPSPLCPhospholipase C gammaPKCParalichthys olivaceusSH3SH2mRNA expression - بیان mRNArapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدOlive flounder - خرچنگ زیتونPhosphoinositide-specific phospholipase C - فسفولیپاز C اختصاص یافته به فسفوینوزیتهlipopolysaccharide - لیپوپلی ساکاریدRace - مسابقهSrc Homology 2 - همبستگی Src 2Src homology 3 - همبستگی Src 3Protein kinase C - پروتئین کیناز سیPoly I:C - پلی I: CPolyinosinic:polycytidylic acid - پلی سونیک: پلیسییدیدیل اسیدcDNA cloning - کلونینگ cDNA
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Phospholipase C gamma 1 and gamma 2 (PLCG1 and PLCG2) are influential in modulating Ca2+ and diacylglycerol, second messengers involved in tyrosine kinase-dependent signaling, including growth factor activation. Here, we used RACE (rapid amplification of cDNA ends) to clone cDNA encoding PLCG1 (PoPLCG1) and PLCG2 (PoPLCG2) in the olive flounder (Paralichthys olivaceus). The respective 1313 and 1249 amino acid sequences share high identity with human PLCG1 and PLCG2, and contain the following domains: pleckstrin homology (PH), EF-hand, catalytic X and Y, Src homology 2 (SH2), Src homology 3 (SH3), and C2. Phylogenic analysis and sequence comparison of PoPLCG1 and PoPLCG2 with other PLC isozymes showed a close relationship between the two PLCGs, supported by structural analysis. In addition, tissue expression analysis showed that PoPLCG1 was expressed predominantly in the brain, eye, and heart, whereas PoPLCG2 was expressed principally in gills, esophagus, spleen, and kidney. Following stimulation with LPS and Poly I:C, PoPLCG expression was compared with the expression of inflammatory cytokines IL-1β, IL-6, and TNF-α via reverse transcription-PCR and real-time quantitative PCR. Our results suggest that PoPLCG isozymes perform a critical immune function in olive flounder, being active in pathogen resistance and the inflammation process.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 63, April 2017, Pages 353-366
Journal: Fish & Shellfish Immunology - Volume 63, April 2017, Pages 353-366
نویسندگان
Soo Ji Woo, Hyae In Jo, Hyung Ho Lee, Joon Ki Chung,