کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5545237 | 1555316 | 2017 | 21 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of Akabane virus from domestic bamboo rat, Southern China
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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چکیده انگلیسی
To identify the causative agents in 3 large-scale outbreaks of encephalitis and death among farmed bamboo rats (Rhizomys pruinosus). The routine bacterial culture and identification were performed. There were no significant pathogenic bacteria isolated from the brain, heart, liver, spleen, lung, or kidney of diseased bamboo rats. Using PCR-based methods, we excluded the following as causative agent: pox virus, herpesvirus, adenovirus, lymphocytic choriomeningitis virus, rabies virus, and sendai virus. Furthermore, the homogenate from the diseased bamboo rats was subjected to viral metagenomic analysis which revealed 48506 filtered viral reads annotated to Akabane virus (AKAV) with >75% nucleotide identity, suggesting the presence of AKAVs in bamboo rats. Five novel AKAV isolates were successfully isolated and characterized. Furthermore the newly isolated AKAV isolate was used to demonstrate that it can reproduce the severe encephalitic and pneumonic disease in bamboo rats and mice. The findings add to the better understanding of AKAV epidemiology and to the prevention and control of Akabane diseases in China.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Microbiology - Volume 207, August 2017, Pages 280-285
Journal: Veterinary Microbiology - Volume 207, August 2017, Pages 280-285
نویسندگان
Hai-bo Tang, Fenglian Chen, Guibo Rao, Anbin Bai, Jiajia Jiang, Yichao Du, Pengfei Ren, Jinfeng Liu, Shaomin Qin, Lei Yang, Jianmin Wu,