Steroids hydroxylation catalyzed by the monooxygenase mutant 139-3 from Bacillus megaterium BM3

Finding new substrates with pharmaceutical potential for biocatalysts like P450 is always challenging. In this contribution, a P450 BM3 mutant 139-3 was observed to exhibit a hydroxylated activity toward androstenedione (1) at 1α-position.135