کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5549565 | 1556733 | 2017 | 7 صفحه PDF | دانلود رایگان |
A rapid, simple and sensitive ultra-high performance liquid chromatography-tandem mass spectrometric (UPLC-MS/MS) method was developed and validated for the determination of hederasaponin B, an active triterpenoid saponin widely existed in Hedera helix L. Plasma samples were processed by protein precipitation with acetonitrile and separated on a Thermo Hypersil GOLD C18 (2.1âmmâÃâ50âmm,1.9âµm) at flow rate of 0.3âml/min, with a gradient elution consisting of acetonitrile and water containing 0.1% (v/v) formic acid at 30â°C and detected by electrospray ionization mass spectrometry in the positive multiple reaction monitoring (MRM) mode. The linearity was found to be within the concentration range of 0.5-5000âng/ml with a lower limit of quantification of 0.5âng/ml. The absolute oral bioavailability of hederasaponin B was 0.24â±â0.49%. This indicated that the concentration-time course of the hederasaponin B existed a double-peak phenomenon. This method was further applied to the determination of hederasaponin B in rat plasma and showed good practicability, for the first time, after intragastric (25âmg/kg) and intravenous (2âmg/kg) administration in rats.
Graphical Abstract25
Journal: Asian Journal of Pharmaceutical Sciences - Volume 12, Issue 4, July 2017, Pages 363-369