کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5551457 1402949 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biogenic nanoparticles bearing antibacterial activity and their synergistic effect with broad spectrum antibiotics: Emerging strategy to combat drug resistant pathogens
ترجمه فارسی عنوان
نانوذرات بیوگرافی حاوی فعالیت ضد باکتریایی و اثر سینرژیک آنها با آنتی بیوتیک های وسیع: استراتژی جدید برای مبارزه با پاتوژن های مقاوم به دارو
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی علوم دارویی
چکیده انگلیسی

The present study emphasizes on synthesis of bimetallic silver-gold nanoparticles from cell free supernatant of Pseudomonas veronii strain AS41G inhabiting Annona squamosa L. The synthesized nanoparticles were characterized using hyphenated techniques with UV-Visible spectra ascertained absorbance peak between 400 and 800 nm. Possible interaction of biomolecules in mediating and stabilization of nanoparticles was depicted with Fourier transform infrared spectroscopy (FTIR). X-ray diffraction (XRD) displayed Bragg's peak conferring the 1 0 0, 1 1 1, 2 0 0, and 2 2 0 facets of the face centered cubic symmetry of nanoparticles suggesting that these nanoparticles were crystalline in nature. Size and shape of the nanoparticles were determined using Transmission electron microscopy (TEM) microgram with size ranging from 5 to 50 nm forming myriad shapes. Antibacterial activity of nanoparticles against significant human pathogens was conferred with well diffusion assay and its synergistic effect with standard antibiotics revealed 87.5% fold increased activity with antibiotic “bacitracin” against bacitracin resistant strains Bacillus subtilis, Escherichia coli and Klebsiella pneumoniae followed by kanamycin with 18.5%, gentamicin with 11.15%, streptomycin with 10%, erythromycin with 9.7% and chloramphenicol with 9.4%. Thus the study concludes with biogenic and ecofriendly route for synthesizing nanoparticles with antibacterial activity against drug resistant pathogens and attributes growing interest on endophytes as an emerging source for synthesis of nanoparticles.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Saudi Pharmaceutical Journal - Volume 25, Issue 1, January 2017, Pages 44-51
نویسندگان
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