کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5551911 1557806 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Passive immunotherapy for Middle East Respiratory Syndrome coronavirus infection with equine immunoglobulin or immunoglobulin fragments in a mouse model
ترجمه فارسی عنوان
ایمونوتراپی منفعل برای عفونت کروناویروس سندرم تنفسی خاورمیانه با قطعات ایمونوگلوبولین اوریون یا ایمونوگلوبولین در یک مدل موش
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
چکیده انگلیسی


- Healthy horses immunized with MERS-CoV virus-like particles rapidly generate high titers of virus neutralizing antibodies.
- Passive transfer of equine immune antibodies significantly reduced virus titers from the lungs of MERS-CoV infected mice.
- F(ab')2 fragments prepared by digestion of antibody with pepsin to reduce possible allergic responses.
- Equine immune antibodies are polyclonal and recognize more antigen determinants in MERS-CoV spike protein.

Middle East Respiratory Syndrome (MERS) is a highly lethal pulmonary infection caused by a coronavirus (CoV), MERS-CoV. With the continuing spread of MERS-CoV, prophylactic and therapeutic treatments are urgently needed. In this study, we prepared purified equine F(ab')2 from horses immunized with MERS-CoV virus-like particles (VLPs) expressing MERS-CoV S, M and E proteins. Both IgG and F(ab')2 efficiently neutralized MERS-CoV replication in tissue culture. Passive transfer of equine immune antibodies significantly reduced virus titers and accelerated virus clearance from the lungs of MERS-CoV infected mice. Our data show that horses immunized with MERS-CoV VLPs can serve as a primary source of protective F(ab')2 for potential use in the prophylactic or therapeutic treatment of exposed or infected patients.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Antiviral Research - Volume 137, January 2017, Pages 125-130
نویسندگان
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