کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5551911 | 1557806 | 2017 | 6 صفحه PDF | دانلود رایگان |
- Healthy horses immunized with MERS-CoV virus-like particles rapidly generate high titers of virus neutralizing antibodies.
- Passive transfer of equine immune antibodies significantly reduced virus titers from the lungs of MERS-CoV infected mice.
- F(ab')2 fragments prepared by digestion of antibody with pepsin to reduce possible allergic responses.
- Equine immune antibodies are polyclonal and recognize more antigen determinants in MERS-CoV spike protein.
Middle East Respiratory Syndrome (MERS) is a highly lethal pulmonary infection caused by a coronavirus (CoV), MERS-CoV. With the continuing spread of MERS-CoV, prophylactic and therapeutic treatments are urgently needed. In this study, we prepared purified equine F(ab')2 from horses immunized with MERS-CoV virus-like particles (VLPs) expressing MERS-CoV S, M and E proteins. Both IgG and F(ab')2 efficiently neutralized MERS-CoV replication in tissue culture. Passive transfer of equine immune antibodies significantly reduced virus titers and accelerated virus clearance from the lungs of MERS-CoV infected mice. Our data show that horses immunized with MERS-CoV VLPs can serve as a primary source of protective F(ab')2 for potential use in the prophylactic or therapeutic treatment of exposed or infected patients.
Journal: Antiviral Research - Volume 137, January 2017, Pages 125-130