کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5557061 | 1560556 | 2017 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Effects of MgSO4 and magnesium transporters on 6-hydroxydopamine-induced SH-SY5Y cells Effects of MgSO4 and magnesium transporters on 6-hydroxydopamine-induced SH-SY5Y cells](/preview/png/5557061.png)
AimsThe magnesium ion (Mg2 +) fulfils several important functions for living organisms. We investigated whether there is a protective effect of MgSO4 on 6-OHDA-induced neurotoxicity in SH-SY5Y cells, and gained insight into the effects of cellular mRNA and protein expression of the magnesium transporters SLC41A1, NIPA1, MagT1 and CNNM2 on 6-OHDA-induced neurotoxicity.Main methodsThe effect of MgSO4 on cell viability in 6-OHDA-treated SH-SY5Y cells was measured using a CCK-8 kit. The mRNA and protein expression of SLC41A1, NIPA1, MagT1, and CNNM2 were detected using reverse transcription-qPCR and Western blot.Key findingsThe results showed that SH-SY5Y cells treated with 25- 50 μM 6-OHDA for 24 h significantly decreased cell viability, while if pre-incubated with 0.125- 1 mM MgSO4 for 1 h before adding 6-OHDA it partially prevented the cell damage. There was a significant decrease in cellular mRNA and protein expression of SLC41A1, NIPA1, MagT1 and CNNM2 in 6-OHDA treated SH-SY5Y cells, and MgSO4 can reverse its decline.SignificanceOur results suggest that MgSO4 may protect SH-SY5Y cells against 6-OHDA-induced cell injury and that gene expression of SLC41A1, NIPA1, MagT1, and CNNM2 might be involved in dopaminergic neurons.
Journal: Life Sciences - Volume 172, 1 March 2017, Pages 48-54