کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5562165 1562596 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Activity of cholinesterases in a young and healthy middle-European population: Relevance for toxicology, pharmacology and clinical praxis
ترجمه فارسی عنوان
فعالیت کولین استراز در یک جمعیت جوان و سالم اروپای اروپایی: اهمیت سم شناسی، فارماکولوژی و پالیسی بالینی
کلمات کلیدی
استیل کولین استراز، بوتیریلولین استراز، ارگانوفسفره مطالعه بالینی، عوامل جمعیتی،
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی


- The modified Ellman's method was used for AChE and BChE assessment in 387 healthy individuals.
- The effect of three main factors (sex, age and smoking) was evaluated.
- Results may be used in military and occupational medicine.
- Reference values for AChE and BChE activity was proposed.

The activity of human cholinesterases, erythrocyte acetylcholinesterase (AChE; EC 3.1.1.7) and plasma butyrylcholinesterase (BChE; EC 3.1.1.8) represents an important marker when monitoring exposure to pesticides/nerve agents, and may also be used in occupational medicine in diagnosis and prognosis of some diseases. In this study “normal/baseline” AChE and BChE activity has been investigated in a young and healthy population, with subsequent evaluation of several intra-population factors including sex, age (categories 18-25, 26-35 and 36-45 years old) and smoker status.The modified Ellman's method was used for enzyme activity assessment in 387 young and healthy individuals (201 males and 186 females aged 18-45). A significant inter-sexual difference in AChE and BChE activity was found (AChE: 351 ± 67 for males and 377 ± 65 for females, (μmol/min)/(μmol of hemoglobin), p < 0.001; BChE: 140 ± 33 for males and 109 ± 29 for females, μkat/l, p < 0.001; mean ± SD). Despite the finding that mean AChE activity somewhat decreased whereas BChE activity grew within the age categories of the tested subjects, no significant effect of age on cholinesterase activity was found (p > 0.05). Smoking influenced cholinesterase activity - AChE activity in smokers was elevated (approx. 3% in males; 8% in females) relative to that in non-smokers (p < 0.05). Smoking was found not to have any effect on BChE activity.Reference values based on confidence intervals for AChE and BChE activity were established. The presented results might be useful in routine clinical practice where the monitoring of blood AChE and plasma BChE activity is crucial for prognosis and diagnosis of organophosphate poisoning, in occupational medicine and in relevant mass casualty scenarios.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology Letters - Volume 277, 5 August 2017, Pages 24-31
نویسندگان
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