کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5562435 1562597 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Induction of proteotoxic stress by the mycotoxin patulin
ترجمه فارسی عنوان
القاء استحکام پروتئوموسیک با میکروسکوپی پاتولین
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی


- The mycotoxin patulin induces the Rpn4 proteotoxic stress response in yeast.
- Induction of Rpn4 protein appears to occur mainly by post-transcriptional mechanisms.
- Rpn4 protein levels are precisely regulated in response to proteotoxic stress.

Patulin is a naturally occurring mycotoxin produced by a number of molds and may contaminate a wide variety of food products. In practice, patulin's main societal relevance concerns apple juice and its products. Multiple advisory bodies, including the U.S. Food and Drug Administration and the World Health Organization, recommend that producers monitor and limit patulin levels in apple juice products. The mechanism of patulin toxicity remains largely unknown. Here we show that patulin induces proteotoxic stress in the yeast S. cerevisiae. The transcription factor Rpn4 controls the abundance of the proteasome, the complex multisubunit protease that destroys proteins, including misfolded proteins. Rpn4 protein is strongly induced by patulin, and Rpn4 levels normalize over time, consistent with homeostatic regulation. A rpn4Δ mutant is highly sensitive to patulin, confirming the physiologic relevance of this response. Rpn4 is known to be regulated both transcriptionally and post-translationally. Patulin induces both pathways of regulation, but the post-transcriptional pathway predominates in controlling Rpn4 protein levels. These results indicate that proteotoxicity represents a major aspect of patulin toxicity. They not only have implications for patulin detoxification but in addition suggest the possibility of some potentially useful patulin applications.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology Letters - Volume 276, 5 July 2017, Pages 85-91
نویسندگان
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