کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5562647 | 1562703 | 2017 | 46 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Acot1 is a sensitive indicator for PPARα activation after perfluorooctanoic acid exposure in primary hepatocytes of Sprague-Dawley rats
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کلمات کلیدی
qPCRPPARαDEGsPFASsACOT1AcotRNA-seqCYP4A1DcfPFOSPFOA2′,7′-dichlorofluorescein - 2 '، 7'-dichlorofluoresceinacyl-CoA thioesterase - acyl-CoA تیو ادراراQuantitative PCR - PCR کمیROS - ROSRNA-sequencing - توالی RNAHADHA - حدیثKEGG یا Kyoto Encyclopedia of Genes and Genomes - دایرة المعارف ژن ها و ژنوم کیوتو Kyoto Encyclopedia of Genes and Genomes - دایره المعارف ژنتیک ژن ها و ژنوم کیوتوPerfluorooctane sulfonate - سولفونات PerfluorooctanePerfluoroalkyl substances - مواد PerfluoroalkylGene ontology - هستیشناسی ژنیHepatocyte - هپاتوسیتPerfluorooctanoic acid - پرفلوئورواکتانوئیک اسیدPeroxisome proliferator activated receptor alpha - پروفیسیم پرولیفراتور فعال گیرنده آلفاDifferentially expressed genes - ژن های متفاوت بیان شده استReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Perfluorooctanoic acid (PFOA) is one of the most commonly detected and persistent perfluoroalkyl substances (PFASs) found in the environment. We found that cell viability and intracellular oxidant stress increased in primary rat hepatocytes exposed to PFOA (100 μM PFOA, 24 h), and mitochondrial superoxide increased from 6.25 μM PFOA treatment group. To screen for sensitive indicators in mRNA level, we investigated global transcriptome profile alteration after PFOA exposure using RNA-sequencing (RNA-seq) in primary rat hepatocytes, and identified 177 gene transcripts (158 upregulated, 19 downregulated) as significantly changed after exposure to 100 μM of PFOA for 24 h (fold change â¥Â 2, FDR < 0.05). Quantitative PCR (qPCR) and RNA-fluorescence in situ hybridization (RNA-FISH) assays were conducted after PFOA treatment at various doses (0, 0.4, 1.56, 6.25, 25, and 100 μM) and times (6, 12, 18, 24, 48, and 96 h). Acot1 transcripts increased significantly in the 100 μM PFOA group (4500-fold) after 24 h of exposure, and increased remarkably for all time points (24, 48, 72 and 96 h) after exposure to 6.25 μM. Acot1 also responded to lower PFOA doses, with a significant increase found after exposure to 0.4 μM for 96 h. These results imply Acot1 could serve as a sensitive indicator for PPARα activation after PFOA exposure in primary rat hepatocytes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 42, August 2017, Pages 299-307
Journal: Toxicology in Vitro - Volume 42, August 2017, Pages 299-307
نویسندگان
Hui Liu, Jianshe Wang, Nan Sheng, Ruina Cui, Yitao Pan, Jiayin Dai,