کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589238 | 1569810 | 2017 | 31 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Digital gene expression profiling of flax (Linum usitatissimum L.) stem peel identifies genes enriched in fiber-bearing phloem tissue
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کلمات کلیدی
CTLreads per kilobase of exon model per million mapped readscellulose synthase-likeNACCOBRACESARG-ICSLRPKMqRT-PCRDEGsDGEFLAbGalCYCEndo-β-1,4-glucanase - Endo-β-1،4-گلوکانازESTs - EST هاLinum usitatissimum L. - Usitatissimum L.beta-galactosidase - بتا گالاکتوزیدازdigital gene expression - بیان ژن دیجیتالcoding sequence - توالی کدگذاریExpressed sequence tags - تگ های توالی بیان شدهKEGG یا Kyoto Encyclopedia of Genes and Genomes - دایرة المعارف ژن ها و ژنوم کیوتو Cellulose synthase - سنتاز سلولزcyclophilin - سیکلوفیلینPhloem - فلوئمBast fiber - فیبر بافتMyb - مبینmyeloblastosis - میلوللاستوزquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیKOR - وقتیDifferentially expressed genes - ژن های متفاوت بیان شده استCdS - کادمیم سولفید، سولفید کادمیمFlax - کتان
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
To better understand the molecular mechanisms and gene expression characteristics associated with development of bast fiber cell within flax stem phloem, the gene expression profiling of flax stem peels and leaves were screened, using Illumina's Digital Gene Expression (DGE) analysis. Four DGE libraries (2 for stem peel and 2 for leaf), ranging from 6.7 to 9.2 million clean reads were obtained, which produced 7.0 million and 6.8 million mapped reads for flax stem peel and leave, respectively. By differential gene expression analysis, a total of 975 genes, of which 708 (73%) genes have protein-coding annotation, were identified as phloem enriched genes putatively involved in the processes of polysaccharide and cell wall metabolism. Differential expression genes (DEGs) was validated using quantitative RT-PCR, the expression pattern of all nine genes determined by qRT-PCR fitted in well with that obtained by sequencing analysis. Cluster and Gene Ontology (GO) analysis revealed that a large number of genes related to metabolic process, catalytic activity and binding category were expressed predominantly in the stem peels. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of the phloem enriched genes suggested approximately 111 biological pathways. The large number of genes and pathways produced from DGE sequencing will expand our understanding of the complex molecular and cellular events in flax bast fiber development and provide a foundation for future studies on fiber development in other bast fiber crops.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 626, 30 August 2017, Pages 32-40
Journal: Gene - Volume 626, 30 August 2017, Pages 32-40
نویسندگان
Yuan Guo, Caisheng Qiu, Songhua Long, Ping Chen, Dongmei Hao, Marta Preisner, Hui Wang, Yufu Wang,