کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589563 | 1569811 | 2017 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
miR-26a and miR-26b inhibit esophageal squamous cancer cell proliferation through suppression of c-MYC pathway
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کلمات کلیدی
eIF2aLDHAmetastasis-associated protein 1MTA1ODCOrnithine decarboxylaseESCCCyclin D1CCND1CDK4c-Myc - c-mycSmall interfering RNA - RNA تداخل کوچکsiRNA - siRNACell proliferation - تکثیر سلولیCAD - طراحی به کمک رایانه یا کَدLactate dehydrogenase A - لاکتات دهیدروژناز ADNA methylation - متیلاسیون DNAUTR یا untranslated regions - منطقه ترجمه نشدهMicroRNA - میکرو RNA MiRNA - میکروRNA، ریزآرانای، miRNAEsophageal squamous cell carcinoma - کارسینوم سلول سنگفرشی مریCOSMIC - کاسمیکcyclin-dependent kinase 4 - کییناز وابسته به سیکلین 4
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Dysregulation of c-Myc is one of the most common abnormalities in human malignancies, including esophageal cancer, one of the world's most lethal cancers. MicroRNA-26 family, including miR-26a and miR-26b, is transcriptionally suppressed by c-MYC. Our previous microarray data indicated a decreased-expression of miR-26 family in esophageal squamous cell carcinoma (ESCC). However, its roles in c-MYC pathway regulation and esophageal cancer tumorigenesis have yet not been elucidated. In this study, we expanded the detection of miR-26 expression in ESCC patients and found that the great majority of ESCC tissues showed an >Â 50% reduction, even in the early-staged tumor. Furthermore, ectopic expression of miR-26a or miR-26b induced ESCC cell growth inhibition and G1 phase arrest. MYC binding protein (MYCBP) was identified as a direct target of miR-26. MiR-26 could dramatically decrease MYCBP mRNA and protein levels, as well as the expression of luciferase carrying MYCBP 3â²-untranslated region. Moreover, knock-down of MYCBP mimicked the effect of miR-26. More importantly, miR-26 overexpression could downregulate a series of c-MYC target genes as MYCBP silence did. Taken together, these results indicate that miR-26 family can suppress esophageal cancer cell proliferation by inhibition of MYCBP, subsequently downregulate c-MYC pathway. Besides, we also found that reduction of miR-26 expression in ESCC was not due to DNA methylation. Hence, our study reveals a novel feedback loop for c-MYC pathway and implicates miR-26 as a potential target for prevention and treatment of esophageal cancer.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 625, 20 August 2017, Pages 1-9
Journal: Gene - Volume 625, 20 August 2017, Pages 1-9
نویسندگان
Juan Li, Yue Liang, Hao Lv, Hui Meng, Gang Xiong, Xingying Guan, Xuedan Chen, Yun Bai, Kai Wang,