کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5590138 | 1570096 | 2016 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification and characterization of microRNAs in maize endosperm response to exogenous sucrose using small RNA sequencing
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Identification and characterization of microRNAs in maize endosperm response to exogenous sucrose using small RNA sequencing Identification and characterization of microRNAs in maize endosperm response to exogenous sucrose using small RNA sequencing](/preview/png/5590138.png)
چکیده انگلیسی
Sucrose acts as a signaling molecule for genes critical to starch biosynthesis in maize endosperm. Previously, we showed that sucrose could regulate starch biosynthesis in maize via transcription factors. To better understand the complex regulation of starch biosynthesis, the 10Â days after pollination endosperm from Zea mays L. B73 inbred line was collected and treated with sucrose for small RNA sequencing. The sequencing results revealed that 24 known miRNAs and 190 novel miRNAs were significantly differentially expressed in response to sucrose. In addition, most of target mRNAs were characterized as transcription factors, mainly including, MYB, ARF, NAC, AP2/ERF, WRKY, and GRAS, which play important roles in starch biosynthesis and seed development in maize endosperm. The expression profiles of miR398a/b and miR159b/j/k followed opposite expression trends to their target genes when analyzed by qPCR. In conclusion, these results show that sucrose regulates the expression of starch synthetic genes through miRNAs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Genomics - Volume 108, Issues 5â6, December 2016, Pages 216-223
Journal: Genomics - Volume 108, Issues 5â6, December 2016, Pages 216-223
نویسندگان
Huanhuan Huang, Jiao Long, Lanjie Zheng, Yangping Li, Yufeng Hu, Guowu Yu, Hanmei Liu, Yinghong Liu, Zhi Huang, Junjie Zhang, Yubi Huang,