|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5599691||1574709||2017||8 صفحه PDF||سفارش دهید||دانلود رایگان|
- Circulating microparticles are increased at high altitude compared to sea level.
- Briefly imposing oscillatory shear impairs endothelial function at high altitude.
- Endothelial function is worsened by endothelial injury at high altitude.
- Brief exposure to high altitude invokes a vulnerable endothelial phenotype.
Background and aimsExposing the endothelium to acute periods of imposed oscillatory shear stress reduces endothelial function and elevates circulating microparticles (MPs). Oscillatory shear stress may be especially pathogenic when superimposed on hypoxia, an environmental stimulus that disrupts the endothelial milieu. We examined the effects of acute manipulation of oscillatory shear stress on endothelial function and circulating MPs at sea level (SL) and high altitude (HA).MethodsHealthy adults (nÂ =Â 12) participated, once at SL and once on the second or third day at HA (3800Â m). Oscillatory shear stress was provoked using a 30-min distal cuff occlusion intervention (75Â mmHg). Endothelial function was assessed before and immediately after the intervention in the brachial artery by reactive hyperaemia flow-mediated dilatation (FMD). Venous blood samples of MPs (flow cytometry) were obtained before and during the last five minutes of the shear intervention.ResultsAt baseline, circulating MPs were two-fold higher at HA (pÂ =Â 0.011) and brachial artery diameter was constricted (pÂ =Â 0.015). Although the intervention at SL increased endothelial-derived MPs by 83Â Â±Â 39% (meanÂ Â±Â SEM; pÂ =Â 0.021), FMD was unaltered. Conversely, at HA, the intervention elicited a 26Â Â±Â 11% reduction in FMD (pÂ =Â 0.020); this reduction was inversely correlated with the change in total circulating MPs (rÂ =Â â0.737, pÂ =Â 0.006) and the change in endothelial-derived MPs (rÂ =Â â0.614, pÂ =Â 0.034).ConclusionsThe vascular endothelium appears to be susceptible to periods of oscillatory shear stress at HA, where impairments in endothelium-dependent vasodilatation may be amplified by endothelial injury. These findings have important implications for understanding the early impact of clinical situations of hypoxaemia on the vascular endothelium.
Journal: Atherosclerosis - Volume 256, January 2017, Pages 115-122