کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
562136 1451899 2013 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
High frequency label-free photoacoustic microscopy of single cells
موضوعات مرتبط
مهندسی و علوم پایه شیمی طیف سنجی
پیش نمایش صفحه اول مقاله
High frequency label-free photoacoustic microscopy of single cells
چکیده انگلیسی

Photoacoustic measurements of melanoma cells and red blood cells (RBCs) using ultra-high frequency (UHF) wide-bandwidth transducers are reported. In this detection system, the resolution typically depends on the parameters of the receiving transducer, and not the focus of the laser. A single melanoma cell was imaged with 200, 375 and 1200 MHz transducers. As the frequency increased, the resolution increased, resulting in greater detail observed. A single RBC was imaged at 1200 MHz, showing the contours of the cell. While lateral and axial resolutions approaching 1 μm are possible with this microscope, the key advantage is the ability to perform a wide-bandwidth quantitative signal analysis of the photoacoustic signals. The power spectrum of the signals measured from RBCs showed distinct spectral minima around 800 and 1500 MHz which are directly related to the RBC geometry. This study reports on the high-resolution imaging capabilities and quantitative analyses using UHF photoacoustic microscopy.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Photoacoustics - Volume 1, Issues 3–4, December 2013, Pages 49–53
نویسندگان
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