کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5666677 | 1591542 | 2017 | 6 صفحه PDF | دانلود رایگان |
- High production rate of ROS in SLE patients derived PMN after fMLP and TPA stimulation.
- Biphasic response profile in SLE patients PMN after fMLP stimulation.
- Difference in PMN oxidative burst between active and remission phase.
- Lipid peroxidation and protein oxidation in SLE PMN lysates.
ObjectiveIncreasing interest is given to the involvement of the innate immunity and especially Polymorphonuclear neutrophils (PMN) in the physiopathological process of inflammatory diseases such as systemic lupus erythematosus (SLE). Here, we investigated the oxidative burst and damages in SLE patients neutrophils, considering the two phases of the disease, the active and the remission/inactive states.MethodsThis study was conducted on 30 SLE patients and 23 healthy controls. The oxidative burst in neutrophils of SLE patients and controls was triggered by fMLP and TPA, while reactive oxygen species (ROS) production was evaluated using a chemiluminescence assay. Oxidative damages in neutrophils were assessed by measuring Free thiol groups level and carbonyl groups, as protein oxidative markers. The malondialdehyde (MDA) level informed about the lipid peroxidation (LPO) and the catalase activity indicated the antioxidant enzymatic activity.ResultCompared to controls, SLE patients exhibited a significantly increased level of ROS production concomitantly to a decreased response time. Their Neutrophils were characterized by a decreased level of MDA and high levels of protein oxidation as evidenced by increased carbonyl groups and decreased SH levels. The catalase activity was higher in SLE patients' neutrophils compared to controls. When patients were clustered according to the disease activity, PMN of patients in active phase showed, paradoxically, a lower ROS production and exhibited higher oxidative damages than the inactive group.ConclusionOur results highlight an altered behavior of LES patients derived PMN particularly in the active phase of the disease. The evaluation of the redox status including the rate of ROS production could be a biological marker to follow the activity of the disease.
Journal: Immunology Letters - Volume 184, April 2017, Pages 1-6