کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5670440 1592665 2017 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Non-neuronal neuropeptide Y and its receptors during acute rejection of rat pulmonary allografts
ترجمه فارسی عنوان
نوروپپتید غیر نورونی و گیرنده های آن در طی رد حاد رگ های رگ ریه رت
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
چکیده انگلیسی


- Neuropeptide Y (NPY) and NPY receptor 1 (Y1) were expressed by pulmonary intravascular and intraalveolar leukocytes in rat.
- Y1 was up-regulated by pulmonary intravascular and intraalveolar leukocytes during allograft rejection.
- An increased leukocytic expression of NPY in lung may results in a reduced accumulation of leukocytes in allograft vessels.

This study tested the hypothesis that neuropeptide Y (NPY) and NPY receptors 1 (Y1) and 2 (Y2) participate in lung allograft rejection. Inflammation in grafts may include interaction between blood leukocytes and graft endothelial cells and marked accumulation of intravascular blood leukocytes. Fewer leukocytes accumulate in lung than in kidney allografts. Lung transplantion was performed in the Dark Agouti to Lewis rat strain combination. Intravascular and intraalveolar leukocytes were isolated from the grafts, and we evaluated the mRNA expression of NPY, Y1, and Y2 by real-time RT-PCR as well as the peptide expression of NPY by radioimmunoassay and immunohistochemistry. NPY and Y1 were expressed by pulmonary intravascular and intraalveolar leukocytes. Y1 was up-regulated by pulmonary intravascular and intraalveolar leukocytes during allograft rejection while Y2 could not be detected. Higher NPY expression levels in intravascular leukocytes were observed in lung compared to kidney allografts, which were investigated previously. Our findings suggest that an increased leukocytic expression of NPY in lung compared to kidney allografts results in a reduced accumulation of leukocytes in allograft vessels.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Transplant Immunology - Volumes 43–44, August 2017, Pages 49-53
نویسندگان
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