کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5671315 | 1592817 | 2017 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: In vitro emergence of fluoroquinolone resistance in Cutibacterium (formerly Propionibacterium) acnes and molecular characterization of mutations in the gyrA gene In vitro emergence of fluoroquinolone resistance in Cutibacterium (formerly Propionibacterium) acnes and molecular characterization of mutations in the gyrA gene](/preview/png/5671315.png)
- Levofloxacin-resistant Cutibacterium acnes mutants can be easily selected in vitro.
- Low- and high-levels resistance can be observed.
- Only GyrA amino-acid changes lead to levofloxacin-resistance.
- S101L substitution is the most frequent one detected with high-level resistance to levofloxacine MIC>32 mg/L.
- Mutants without any mutation may express efflux pump system.
In vitro occurrence of levofloxacin (LVX) resistance in C. acnes and characterization of its molecular background were investigated. The mutation frequency was determined by inoculation of 108 cfu of C. acnes ATCC 11827 (LVX MIC = 0.25 mg/L) on LVX-containing agar plates. The progressive emergence of resistance was studied by a second exposure to increasing LVX concentrations. For mutants, the QRDR regions including the gyrA and parC genes were sequenced and compared to both C. acnes ATCC 11827 and C. acnes KPA171202 reference sequences (NC006085). The importance of the efflux pump system in resistance was investigated by using inhibitors on selected resistant mutants with no mutation in the QRDR. C. acnes growth was observed on LVX-containing plates with mutation frequencies of 3.â8 cfu Ã 10â8 (8 Ã MIC) and 1.6 cfu Ã 10â7 (4 Ã MIC). LVX resistance emerged progressively after one-step or two-step assays. In LVX-resistant isolates, the MIC ranged from 0.75 to >32 mg/L. Mutations were detected exclusively in the gyrA gene. Ten genotypes were identified: G99âC, G99âD, D100N, D100âH, D100âG, S101L, S101W, A102âP, D105âH and A105âG. Mutants S101L and S101W were always associated with a high level of resistance. Mutants with no mutation in the QRDR were more susceptible when incubated with an efflux pump inhibitor (phenyl-arginine β-naphthylamide) only, suggesting, for the first time, the expression of such a system in C. acnes LVX-resistant mutants.
Journal: Anaerobe - Volume 47, October 2017, Pages 194-200