کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5673104 | 1593436 | 2017 | 26 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cytotoxicity and antiviral activity of electrochemical - synthesized silver nanoparticles against poliovirus
ترجمه فارسی عنوان
سمیت سلولی و فعالیت ضد ویروسی نانوذرات نقره سنتز شده الکتروشیمیایی علیه ویروس
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
چکیده انگلیسی
Silver nanoparticles (AgNPs) have been proven to have noticeable cytotoxicity in vitro and antiviral activity against some types of enveloped viruses. This paper presents the cytotoxicity and antiviral activity of pure AgNPs synthesized by the electrochemical method, towards cell culture and poliovirus (a non-enveloped virus). Prepared AgNPs were characterized by ultraviolet-visible spectroscopy, energy-dispersive X-ray spectroscopy and transmission electron microscopy. Before incubation with poliovirus, different concentrations of AgNPs were added to human rhabdomyosarcoma (RD) cell monolayers seeded in 96 well plates for testing their cytotoxicity. The in vitro cytotoxicity and anti-poliovirus activity of AgNPs were daily assessed for cytopathic effect (CPE) through inverted light microscopy. CPE in the tested wells was determined in comparison with those in wells of negative and positive control. Structure analysis showed that AgNPs were formed with a quasi-spherical shape with mean size about 7.1Â nm and high purity. No CPE of RD cells was seen in wells at the time point of 48Â h post-incubation with AgNPs at concentration up to 100Â ppm. The anti-poliovirus activity of AgNPs was determined at 3.13Â ppm corresponding to the viral concentration of 1TCID50 (Tissue Culture Infective Dose) after 30Â min, and 10TCID50 after 60Â min, the cell viability was found up to 98% at 48Â h post-infection, with no CPE found. Whereas, a strong CPE of RD cells was found at 48Â h post-infection with the mixture of AgNPs and poliovirus at concentration of 100TCID50, and in wells of positive controls. With mentioned advantages, electrochemical-synthesized AgNPs are promising candidate for advanced biomedical and disinfection applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 241, March 2017, Pages 52-57
Journal: Journal of Virological Methods - Volume 241, March 2017, Pages 52-57
نویسندگان
Tran Quang Huy, Nguyen Thi Hien Thanh, Nguyen Thanh Thuy, Pham Van Chung, Pham Ngoc Hung, Anh-Tuan Le, Nguyen Thi Hong Hanh,