کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5673135 1593427 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Differentiation of minute virus of mice and mouse parvovirus by high resolution melting curve analysis
ترجمه فارسی عنوان
تنوع ویروس موش و موش پاروویروس موش با تجزیه و تحلیل منحنی ذوب با وضوح بالا
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
چکیده انگلیسی


- A simple, sensitive, specific PCR-HRM assay was established for murine parvovirus.
- The method differentiates MVM and MPV strains in one reaction tube.
- Tissue samples and co-infection assays were tested to verify the method.

Murine parvovirus is one of the most prevalent infectious pathogens in mouse colonies. A specific primer pair targeting the VP2 gene of minute virus of mice (MVM) and mouse parvovirus (MPV) was utilized for high resolution melting (HRM) analysis. The resulting melting curves could distinguish these two virus strains and there was no detectable amplification of the other mouse pathogens which included rat parvovirus (KRV), ectromelia virus (ECT), mouse adenovirus (MAD), mouse cytomegalovirus (MCMV), polyoma virus (Poly), Helicobactor hepaticus (H. hepaticus) and Salmonella typhimurium (S. typhimurium). The detection limit of the standard was 10 copies/μL. This study showed that the PCR-HRM assay could be an alternative useful method with high specificity and sensitivity for differentiating murine parvovirus strains MVM and MPV.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 250, December 2017, Pages 41-46
نویسندگان
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