کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5673747 | 1593681 | 2017 | 8 صفحه PDF | دانلود رایگان |
- Effect of static magnetic field on fatty acid composition.
- Analysis of different proportions of fatty acids.
- Expression of cfa gene encoding for cyclopropane fatty acid synthase.
- Effect on membrane fluidity.
We have been interested, in this work, to investigate the effect of the exposure to static magnetic field at 200Â mT (SMF) on the fatty acid (FA) composition of Salmonella enterica subsp Enterica serovar Hadar isolate 287: effects on the proportion of saturated and unsaturated fatty acids (SFAs, UFAs), cyclopropane fatty acids (CFAs) and hydroxy fatty acids after exposure to the static magnetic field at 200Â mT (SMF). Analysis with Gas Chromatography-Mass Spectrometry (GC-MS) of total lipid showed that the proportion of the most fatty acids was clearly affected. The comparison of UFAs/SFAs ratio in exposed bacteria and controls showed a diminution after 3 and 6Â h of exposure. This ration reached a balance after 9Â h of treatment with SMF. So we can conclude that S. Hadar tries to adapt to magnetic stress by changing the proportions of SFAs and UFAs over time to maintain an equilibrium after 9Â h of exposure, thus to maintain the inner membranes fluidity. Also, a decrease in the proportion of hydroxy FAs was observed after 6Â h but an increase of this proportion after 9Â h of exposure. Concerning CFAs, its proportion raised after 6Â h of exposure to the SMF but it decreased after 9Â h of exposure. These results are strongly correlated with those of cfa (cyclopropane fatty acid synthase) gene expression which showed a decrease of its expression after 9Â h of exposure.
Journal: Microbial Pathogenesis - Volume 108, July 2017, Pages 13-20