کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5675121 1594217 2017 16 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simulation of the M13 life cycle I: Assembly of a genetically-structured deterministic chemical kinetic simulation
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Simulation of the M13 life cycle I: Assembly of a genetically-structured deterministic chemical kinetic simulation
چکیده انگلیسی


- Available quantitative data on M13 biology assembled as a chemical kinetic simulation.
- Model reproduces holistic behavior of infection from DNA entry to progeny release.
- Simulation matches experimentally-measured DNA, RNA, protein and progeny levels.

To expand the quantitative, systems level understanding and foster the expansion of the biotechnological applications of the filamentous bacteriophage M13, we have unified the accumulated quantitative information on M13 biology into a genetically-structured, experimentally-based computational simulation of the entire phage life cycle. The deterministic chemical kinetic simulation explicitly includes the molecular details of DNA replication, mRNA transcription, protein translation and particle assembly, as well as the competing protein-protein and protein-nucleic acid interactions that control the timing and extent of phage production. The simulation reproduces the holistic behavior of M13, closely matching experimentally reported values of the intracellular levels of phage species and the timing of events in the M13 life cycle. The computational model provides a quantitative description of phage biology, highlights gaps in the present understanding of M13, and offers a framework for exploring alternative mechanisms of regulation in the context of the complete M13 life cycle.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 500, January 2017, Pages 259-274
نویسندگان
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