کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5675235 | 1594207 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular characterization of AID-mediated reduction of hepatitis B virus transcripts
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کلمات کلیدی
nESActivation-induced cytidine deaminasepgRNArcDNArelaxed circular DNAAPOBECTGF-βpregenomic RNA - RNA pregenomicimmunoglobulin - ایمونوگلوبولینClass switch - سوئیچ کلاسnuclear export signal - سیگنال صادرات هسته ایwild type - نوع وحشیHBV - هپاتیت بhepatitis B virus - ویروس هپاتیت بیAntiviral protein - پروتئین ضد ویروسیAID - کمک
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Hepatitis B virus (HBV) is the major cause of liver cirrhosis and hepatocellular carcinoma. After entering a hepatocyte, HBV forms a nuclear viral episome and produces pregenomic (pg) RNA with a stem-loop structure called an epsilon, which acts to signal encapsidation. We previously demonstrated that TGF-β upregulates activation-induced cytidine deaminase (AID) expression in hepatocytes, which in turn downregulates HBV transcripts by recruiting the RNA exosome complex. The molecular mechanism underlying AID-mediated HBV RNA reduction remains largely unclear. Here we used a pgRNA reporter system having a reporter gene within pgRNA to identify sis- and trans-acting elements in AID-mediated HBV RNA reduction. We found that the epsilon RNA and C-terminus of AID are required for AID-mediated HBV RNA reduction. Importantly, this reduction was reproduced in a hydrodynamic HBV transfection mouse model. The molecular mechanism of AID-mediated HBV RNA reduction is discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 510, October 2017, Pages 281-288
Journal: Virology - Volume 510, October 2017, Pages 281-288
نویسندگان
Lusheng Que, Guangyan Liu, Kouichi Kitamura, Kousho Wakae, Yingfang Li, Hironori Nishitsuji, Saneyuki Ujino, Kunitada Shimotohno, Masamichi Muramatsu,