کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5675531 | 1594320 | 2017 | 10 صفحه PDF | دانلود رایگان |
- The exact 5â² and 3â² ends of already know transcripts determined.
- Two novel 3â²-UTR and a non-spliced isoform of Cap discovered.
- Novel 3â²-UTR and 5â²-UTR isoforms and splice variant discovered for Rep and Rep'.
- New genome spanning long transcripts with a new splice site identified.
- The transcript for the putative ORF3 discovered.
In this study, we used Pacific Biosciences RS II long-read and Illumina HiScanSQ short-read sequencing technologies for the characterization of porcine circovirus type 1 (PCV-1) transcripts. Our aim was to identify novel RNA molecules and transcript isoforms, as well as to determine the exact 5â²- and 3â²-end sequences of previously described transcripts with single base-pair accuracy. We discovered a novel 3â²-UTR length isoform of the Cap transcript, and a non-spliced Cap transcript variant. Additionally, our analysis has revealed a 3â²-UTR isoform of Rep and two 5â²-UTR isoforms of Rep' transcripts, and a novel splice variant of the longer Rep' transcript. We also explored two novel long transcripts, one with a previously identified splice site, and a formerly undetected mRNA of ORF3. Altogether, our methods have identified nine novel RNA molecules, doubling the size of PCV-1 transcriptome that had been known before. Additionally, our investigations revealed an intricate pattern of transcript overlapping, which might produce transcriptional interference between the transcriptional machineries of adjacent genes, and thereby may potentially play a role in the regulation of gene expression in circoviruses.
Journal: Virus Research - Volume 237, 2 June 2017, Pages 37-46