کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5704081 | 1602569 | 2016 | 50 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Involvement of P2X7 receptors in retinal ganglion cell apoptosis induced by activated Müller cells
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کلمات کلیدی
PBSphosphatidylinositol-phospholipase Cchronic ocular hypertensionPI-PLCBBGBrilliant Blue Goxidized ATPoxATPmGluRFluorogoldPKCiOpDHPGGFAPpKaRGC(S)-3,5-dihydroxyphenylglycine - (S) -3،5-دی هیدروکسی فنیل گلیسینApoptosis - خزان یاختهایCNS - دستگاه عصبی مرکزیdiacylglycerol - دیسیل گلیسیرینDAG - روزretinal ganglion cell - سلول گانگلیونی شبکیهcentral nervous system - سیستم عصبی مرکزیphosphate buffer saline - فسفات بافر شورIntraocular pressure - فشار داخل چشمGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالprotein kinase A - پروتئین کیناز AProtein kinase C - پروتئین کیناز سیCOH - کوهGlutamine synthetase - گلوتامین سنتتازGlaucoma - گلوکوم P2X7 receptor - گیرنده P2X7Metabotropic glutamate receptor - گیرنده گلوتامات متابوتروپیک
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی و میکروب شناسی (عمومی)
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چکیده انگلیسی
Müller cell reactivation (gliosis) is an early response in glaucomatous retina. Previous studies have demonstrated that activation of P2X7 receptors results in retinal ganglion cell (RGC) apoptosis. Here, the issues of whether and how activated Müller cells may contribute to RGC apoptosis through P2X7 receptors were investigated. Either intravitreal injection of (S)-3,5-dihydroxyphenylglycine (DHPG), a group I metabotropic glutamate receptor (mGluR I) agonist, in normal rat retinas, or DHPG treatment of purified cultured rat retinal Müller cells induced an increase in glial fibrillary acidic protein (GFAP) expression, indicative of Müller cell gliosis. In addition, an increase in adenosine triphosphate (ATP) release from purified cultured Müller cells was detected during DHPG treatment (for 10Â min to 48Â h), which was mediated by the intracellular mGluR5/Gq/PI-PLC/PKC signaling pathway. Intravitreal injection of DHPG mimicked the reduction in the number of fluorogold retrogradely labeled RGCs in chronic ocular hypertension (COH) rats. Treatment with the conditioned culture medium (CM) obtained from the DHPG-activated Müller cell medium induced an increase in the number of TUNEL-positive cells in cultured RGCs, which was mimicked by benzoylbenzoyl adenosine triphosphate (BzATP), a P2X7 receptor agonist, but was partially blocked by brilliant blue G (BBG), a P2X7 receptor antagonist. Moreover, the CM treatment of cultured RGCs significantly increased Bax protein level and decreased Bcl-2 protein level, which was also mimicked by BzATP and partially blocked by BBG, respectively. These results suggest that reactivated Müller cells may release excessive ATP, in turn leading to RGC apoptosis through activating P2X7 receptors in these cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Eye Research - Volume 153, December 2016, Pages 42-50
Journal: Experimental Eye Research - Volume 153, December 2016, Pages 42-50
نویسندگان
Bo Xue, Yuting Xie, Ying Xue, Nan Hu, Guowei Zhang, Huaijin Guan, Min Ji,