کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5737463 | 1614724 | 2017 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Botulinum neurotoxin type A-cleaved SNAP25 is confined to primary motor neurons and localized on the plasma membrane following intramuscular toxin injection
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کلمات کلیدی
PBSGFAPDASMOAVAChTTeNTVGLUT2GADVGLUT1MNTPABSNARESNAP25NMJOnabotulinumtoxinA2 dimensional - 2 بعدیBoNT/A - BoNT / Aglutamic acid decarboxylase - glutamic acid dearboxylaseTibialis Anterior - Tibialis قدامیPolyclonal antibody - آنتی بادی های پلی کلونالNeuromuscular junction - اتصال عصبی عضلانیOct - اکتبرtyrosine hydroxylase - تیروزین هیدروکسیلازvesicular acetylcholine transporter - حمل کننده استیل کولین vesicularvesicular glutamate transporter 2 - حمل کننده گلوتامات vesicular 2vesicular glutamate transporter 1 - حمل کننده گلوتامات وزیکولار 1CNS - دستگاه عصبی مرکزیoptimal cutting temperature - دمای برش مطلوبTetanus toxin - سموم تتراcentral nervous system - سیستم عصبی مرکزیSpinal cord - طناب نخاعیPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریMechanism of action - مکانیسم عملBotulinum neurotoxin - نوروتوکسین بوتولینومMotor neuron - نورون حرکتیbotulinum neurotoxin type A - نوع نوروتوکسین بوتولینوم نوع AMotor nerve terminals - پایانه های عصبی موتورGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالChAT - چتcholine acetyltransferase - کولین استیل ترانسفرازsoluble N-ethylmaleimide-sensitive factor attachment protein receptor - گیرنده پروتئین دلبستگی حساس به پروتئین محلول N-ethylmaleimide
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The mechanism of action of botulinum neurotoxin type A (BoNT/A) is well characterized, but some published evidence suggests the potential for neuronal retrograde transport and cell-to-cell transfer (transcytosis) under certain experimental conditions. The present study evaluated the potential for these processes using a highly selective antibody for the BoNT/A-cleaved substrate (SNAP25197) combined with 3-dimensional imaging. SNAP25197 was characterized in a rat motor neuron (MN) pathway following toxin intramuscular injections at various doses to determine whether SNAP25197 is confined to MNs or also found in neighboring cells or nerve fibers within spinal cord (SC). Results demonstrated that SNAP25197 immuno-reactive staining was colocalized with biomarkers for MNs, but not with markers for neighboring neurons, nerve fibers or glial cells. Additionally, a high dose of BoNT/A, but not a lower dose, resulted in sporadic SNAP25197 signal in distal muscles and associated SC regions without evidence for transcytosis, suggesting that the staining was due to systemic spread of the toxin. Despite this spread, functional effects were not detected in the distal muscles. Therefore, under the present experimental conditions, our results suggest that BoNT/A is confined to MNs and any evidence of distal activity is due to limited systemic spread of the toxin at higher doses and not through transcytosis within SC. Lastly, at higher doses of BoNT/A, SNAP25197 was expressed throughout MNs and colocalized with synaptic markers on the plasma membrane at 6 days post-treatment. These data support previous studies suggesting that SNAP25197 may be incorporated into SNARE-protein complexes within the affected MNs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 352, 3 June 2017, Pages 155-169
Journal: Neuroscience - Volume 352, 3 June 2017, Pages 155-169
نویسندگان
Brian B. Cai, Joseph Francis, Mitchell F. Brin, Ron S. Broide,