کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5737635 | 1614718 | 2017 | 56 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Distinctive features of Phox2b-expressing neurons in the rat reticular formation dorsal to the trigeminal motor nucleus
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
CCHSpostsynaptic currentsGLYT2pFRGVGLUT1VGLUT2GAD65INaPmedialRTNEYFPglutamic acid decarboxylase 65PRVPHOX2BCcellGmaxPSCsPBNRDVMOVSTIMstimulating electrodeVRestsupratrigeminal nucleusGad67recSCPDAPITBS4-APglutamic acid decarboxylase 67eGFPAMPDAB3,3′-diaminobenzidine tetrahydrochloride - 3،3'-diaminobenzidine tetrahydrochloride4-aminopyridine - 4-آمینوپیریدین4,6-diamidino-2-phenylindole - 4،6-دیامیدین-2-فنیلینولKolliker–Fuse nucleus - Kolliker - هسته فیوزBAC - LACsuperior cerebellar peduncle - اسب سواری مخچه بزرگMED - باphosphate buffer - بافر فسفاتafterhyperpolarization - بعد از پمپاژTris-buffered saline - تریس بافر شورMastication - جویدنvesicular glutamate transporter 2 - حمل کننده گلوتامات vesicular 2vesicular glutamate transporter 1 - حمل کننده گلوتامات وزیکولار 1cell capacitance - خازن سلولیAmplitude - دامنه(فیزیک)DOR - دردpostnatal day - روز پس از زایمانCongenital central hypoventilation syndrome - سندرم hypoventilation مرکزی مادرزادیSuckling - سوزشdorsal - عقبAHP - فرایند تحلیل سلسلهمراتبیinput resistance - مقاومت ورودیpremotor neuron - نورون پیش رانConductance - هدایتparabrachial nucleus - هسته parabrachialPrincipal sensory trigeminal nucleus - هسته ترشحه اصلی حسیMesencephalic trigeminal nucleus - هسته سه هسته ای MesencephalicTrigeminal motor nucleus - هسته موتور دردیRetrotrapezoid nucleus - هسته ی Retrotrapezoidaction potential - پتانسیل عمل resting membrane potential - پتانسیل غشای استراحتreversal potential - پتانسیل معکوسenhanced yellow fluorescent protein - پروتئین فلورسنت زرد افزایش یافته استenhanced green fluorescent protein - پروتئین فلورسنت سبز افزایش یافته استPatch clamp - پچ گیرbacterial artificial chromosome - کروموزوم مصنوعی باکتریاییparafacial respiratory group - گروه تنفسی پارافاقیglycine transporter 2 - گلیسین حمل کننده 2Erev - یورو
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Phox2b encodes a paired-like homeodomain-containing transcription factor essential for development of the autonomic nervous system. Phox2b-expressing (Phox2b+) neurons are present in the reticular formation dorsal to the trigeminal motor nucleus (RdV) as well as the nucleus of the solitary tract and parafacial respiratory group. However, the nature of Phox2b+ RdV neurons is still unclear. We investigated the physiological and morphological properties of Phox2b+ RdV neurons using postnatal day 2-7 transgenic rats expressing yellow fluorescent protein under the control of Phox2b. Almost all of Phox2b+ RdV neurons were glutamatergic, whereas Phox2b-negative (Phox2bâ) RdV neurons consisted of a few glutamatergic, many GABAergic, and many glycinergic neurons. The majority (48/56) of Phox2b+ neurons showed low-frequency firing (LF), while most of Phox2bâ neurons (35/42) exhibited high-frequency firing (HF) in response to intracellularly injected currents. All, but one, Phox2b+ neurons (55/56) did not fire spontaneously, whereas three-fourths of the Phox2bâ neurons (31/42) were spontaneously active. K+ channel and persistent Na+ current blockers affected the firing of LF and HF neurons. The majority of Phox2b+ (35/46) and half of the Phox2bâ neurons (19/40) did not respond to stimulations of the mesencephalic trigeminal nucleus, the trigeminal tract, and the principal sensory trigeminal nucleus. Biocytin labeling revealed that about half of the Phox2b+ (5/12) and Phox2bâ RdV neurons (5/10) send their axons to the trigeminal motor nucleus. These results suggest that Phox2b+ RdV neurons have distinct neurotransmitter phenotypes and firing properties from Phox2bâ RdV neurons and might play important roles in feeding-related functions including suckling and possibly mastication.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 358, 1 September 2017, Pages 211-226
Journal: Neuroscience - Volume 358, 1 September 2017, Pages 211-226
نویسندگان
Kouta Nagoya, Shiro Nakamura, Keiko Ikeda, Hiroshi Onimaru, Atsushi Yoshida, Kiyomi Nakayama, Ayako Mochizuki, Masaaki Kiyomoto, Fumihiko Sato, Kiyoshi Kawakami, Koji Takahashi, Tomio Inoue,