کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5746807 1618801 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Phytoestrogens and sterols in waters with cyanobacterial blooms - Analytical methods and estrogenic potencies
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
Phytoestrogens and sterols in waters with cyanobacterial blooms - Analytical methods and estrogenic potencies
چکیده انگلیسی


- Flavonoids detected in stagnant water bodies in ng/L, sterols in μg/L.
- Relative estrogenic potencies 8 studied flavonoids ranged from 2.2E-5 to 1.3E-3.
- No estrogenic activity observed for 6 selected sterols.
- Estrogenic equivalent of water samples from stagnant water bodies up to 2 ng/L.
- Minor contribution of phytoestrogens to the estrogenic activity of water samples.

Compounds with estrogenic potencies and their adverse effects in surface waters have received much attention. Both anthropogenic and natural compounds contribute to overall estrogenic activity in freshwaters. Recently, estrogenic potencies were also found to be associated with cyanobacteria and their blooms in surface waters. The present study developed and compared the solid phase extraction and LC-MS/MS analytical approaches for determination of phytoestrogens (8 flavonoids - biochanin A, coumestrol, daidzein, equol, formononetin, genistein, naringenin, apigenin - and 5 sterols - ergosterol, β-sitosterol, stigmasterol, campesterol, brassicasterol) and cholesterol in water. The method was used for analyses of samples collected in stagnant water bodies dominated by different cyanobacterial species. Concentrations of individual flavonoids ranged from below the limit of detection to 3.58 ng/L. Sterols were present in higher amounts up to 2.25 μg/L. Biological potencies of these phytoestrogens in vitro were characterized using the hERα-HeLa-9903 cell line. The relative estrogenic potencies (compared to model estrogen - 17β-estradiol) of flavonoids ranged from 2.25E-05 to 1.26E-03 with coumestrol being the most potent. None of the sterols elicited estrogenic response in the used bioassay. Estrogenic activity was detected in collected field water samples (maximum effect corresponding to 2.07 ng/L of 17β-estradiol equivalents, transcriptional assay). At maximum phytoestrogens accounted for only 1.56 pg/L of 17β-estradiol equivalents, contributing maximally 8.5% of the total estrogenicity of the water samples. Other compounds therefore, most likely of anthropogenic origin such as steroid estrogens, are probably the major drivers of total estrogenic effects in these surface waters.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemosphere - Volume 170, March 2017, Pages 104-112
نویسندگان
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