Changes in nutrient removal and flocs characteristics generated by presence of ZnO nanoparticles in activated sludge process

The aim of this work was to evaluate the impact generated by ZnO NPs on the activated sludge process treating raw (RWW) and filtered wastewater (FWW). It was analyzed the oxygen uptake rate, nutrient removal, flocs characteristics and the morphological interactions between activated sludge and ZnO NPs, in presence of 450-2000 mg/L. The results showed that the presence of more than 450 mg/L of ZnO NPs in raw and filtered wastewater inhibited the oxygen uptake by activated sludge. The highest inhibition was 35% in presence of 1500 mg/L in RWW. The organic matter removal was only inhibited in the presence of 450 and 900 mg/L of ZnO NPs; while ammonia removal decreased for all concentrations of ZnO NPs in both types of wastewater, around 13% for RWW and up to 9% for FWW. The orthophosphate removal improved as the concentration of ZnO NPs increased for both wastewater types, enhancing up to 8% for RWW and 17% for FWW. The flocs size of activated sludge exposed to ZnO NPs in RWW decreased as the concentration of ZnO NPs increased; while for FWW, an opposite effect was observed. The elemental mapping allowed detect the Zn inside of microorganisms, which may correspond to a toxicity mechanism in RWW and FWW. These results indicated that the changes in nutrient removal and flocs characteristics caused by the presence of ZnO NPs on the activated sludge are related to wastewater characteristics, such as suspended solids, type of substrate and concentration of ZnO NPs.