White clover living mulch reduces the need for phosphorus fertilizer application to corn

White clover living mulch (LM) increases the uptake of phosphorus (P) and the yield of the main crop by promoting the colonization of arbuscular mycorrhiza (AM). However, the extent to which the P fertilizer application rate can be reduced by using LM is not yet known. This study aimed to address this question. Two field experiments were conducted from 2008 to 2009 (Experiment 1) and from 2009 to 2010 (Experiment 2) at the fields where the available P of soil fluctuated near the lower limit of the optimum P level (43.6 mg kg−1: Truog method). Experiment 1 had a randomized block design, and Experiment 2 had a split-plot design with a factorial arrangement of two cropping systems (LM and no LM) with three P application treatments (0 kg ha−1, 43.6 kg ha−1, and 87.3 kg ha−1). LM increased P concentrations in the early stages of growth and the yield of corn. This can be attributed to the increased AM colonization rate in the early stages of growth. The yield and total digestible nutrient yield of corn in LM with no P application was comparable to the maximum yield in no LM with or without P application. Therefore, LM could make unnecessary P fertilization in soils where P fertilization is required for silage corn.