کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5767289 1628380 2018 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular identification of species and production origins of edible bird's nest using FINS and SYBR green I based real-time PCR
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Molecular identification of species and production origins of edible bird's nest using FINS and SYBR green I based real-time PCR
چکیده انگلیسی


- New insight into differentiation of EBN from houses and caves via molecular approach.
- FINS and SYBR green I based real-time PCR were used for EBN identification.
- Cyt b, ND2, 12S and Fib7 were useful gene markers to identify EBN origins.
- FINS unambiguously identified EBN via swiftlet species and production origins.
- Rapid detection of genuine EBN using SYBR green I based real-time PCR.

The increasing demand and consumption of edible bird's nest (EBN) by people worldwide has contributed to the food fraud issue. To ensure the authenticity of EBN in regard to their origin, rapid and accurate analytical methods are very much needed. In this study, forensically informative nucleotide sequencing (FINS) technique based on mitochondrial and nuclear DNA sequences, and phylogenetic analysis was performed to identify the species and production origins of raw and commercial EBNs. The cytochrome b (Cyt b), NADH dehydrogenase subunit 2 (ND2), 12S ribosomal RNA and beta-fibrinogen intron 7 gene markers used were able to identify and classify EBN produced by Aerodramus fuciphagus and Aerodramus maximus. It was newly discovered that EBN from man-made houses and natural caves were genetically differentiable using the mitochondrial Cyt b and ND2 genes. The phylogenetic results revealed that all EBN samples were well-separated into two groups following their species origin and production origin. A rapid and cost-effective identification alternative of SYBR green I based real-time PCR assay targeting a 177 bp of the mitochondrial Cyt b gene was developed and it efficiently differentiated genuine EBN from counterfeits. This FINS and SYBR green I based real-time PCR are highly sensitive, specific and reliable methods for identification of EBN origins and could be useful for preventing fraud substitution and mislabelling of EBN to ensure food safety.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Control - Volume 84, February 2018, Pages 118-127
نویسندگان
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