کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5768473 | 1628517 | 2017 | 8 صفحه PDF | دانلود رایگان |
- Shelf-life of beef depends on bacterial contamination and storage temperature.
- Use of a uniform shelf-life for fresh beef can result in spoilage before expiration.
- The growth of spoilage bacteria on beef was mathematically predicted.
- The shelf-life charts of beef were developed based on mathematical modeling.
- The shelf-life chart may be helpful to manage the safety and quality of beef.
Shelf-life charts of beef according to bacterial contamination and storage temperature were developed via mathematical regressions of the growth curves of spoilage bacteria in beef. Additionally, bacterial contamination in retail beef specimens was investigated using culture-based bacteriological tests, PCR assays, and pyrosequencing analysis. Pathogenic bacteria were infrequently detected in the 100 retail beef specimens, but spoilage bacteria were found in all of the specimens. The populations of spoilage bacteria in retail beef specimens varied from 2.27 to 6.15 log CFU·gâ1. Pyrosequencing analysis of retail beef specimens suggested contamination from multiple sources, since both Lactobacillales and Pseudomonadales, which are prevalent in bovine intestine and feces and in bovine hides and beef-processing facilities, respectively, were detected. Growth of spoilage bacteria in beef at 5-25 °C was predicted via mathematical characterization of experimentally determined growth curves at 5, 10, 15, 20, and 25 °C using a modified-Gompertz function and subsequent nonlinear regressions of the growth characteristics, and then transformed to shelf-life charts according to the level of bacterial contamination and storage temperature. The bacterial contamination and bacteriological standards used to generate the shelf-life charts were 0, 1, 2, 3, 4, and 5 log CFU·gâ1 and 5, 6, 7, and 8 log CFU·gâ1, respectively.
Journal: LWT - Food Science and Technology - Volume 81, August 2017, Pages 50-57