کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5799625 1555331 2016 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sequence adaptations during growth of rescued classical swine fever viruses in cell culture and within infected pigs
ترجمه فارسی عنوان
سازگاری های توالی در طول رشد ویروس های کلاسیک سرخک خوک در کشت سلولی و در خوک های آلوده
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی


- CSFVs were rescued from full-length cloned cDNAs and passaged in cell culture.
- Specific adaptations occurred within the virus coding sequence in cell culture.
- The same virus adaptations in two proteins occurred during infection of swine.
- Modifications to the CSFV IRES attenuated the virus in swine.
- Swine infected with these attenuated viruses seroconverted against CSFV.

Classical swine fever virus (CSFV) causes an economically important disease of swine. Four different viruses were rescued from full-length cloned cDNAs derived from the Paderborn strain of CSFV. Three of these viruses had been modified by mutagenesis (with 7 or 8 nt changes) within stem 2 of the subdomain IIIf of the internal ribosome entry site (IRES) that directs the initiation of protein synthesis. Rescued viruses were inoculated into pigs. The rescued vPader10 virus, without modifications in the IRES, induced clinical disease in pigs that was very similar to that observed previously with the parental field strain and transmission to in-contact pigs occurred. Two sequence reversions, in the NS2 and NS5B coding regions, became dominant within the virus populations in these infected pigs. Rescued viruses, with mutant IRES elements, did not induce disease and only very limited circulation of viral RNA could be detected. However, the animals inoculated with these mutant viruses seroconverted against CSFV. Thus, these mutant viruses were highly attenuated in vivo. All 4 rescued viruses were also passaged up to 20 times in cell culture. Using full genome sequencing, the same two adaptations within each of four independent virus populations were observed that restored the coding sequence to that of the parental field strain. These adaptations occurred with different kinetics. The combination of reverse genetics and in depth, full genome sequencing provides a powerful approach to analyse virus adaptation and to identify key determinants of viral replication efficiency in cells and within host animals.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Microbiology - Volume 192, 30 August 2016, Pages 123-134
نویسندگان
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