کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5799773 | 1555340 | 2016 | 5 صفحه PDF | دانلود رایگان |
- A goose parvovirus related parvovirus was isolated from diseased duck with beak atrophy and dwarfism syndrome.
- The pathogenicity, pathogenesis, viral shedding, and immune responses were generated in our BADS experiment.
- The methods will help establish an experimental model to evaluate the potential role of ducks in BADS epidemiology.
Infection of clinically susceptible ducks, including cherry valley and Muscovy ducks, with a novel goose parvovirus (GPV)-related virus (N-GPV) can result in beak atrophy and dwarfism syndrome (BADS). To obtain new insights into the host range and pathogenic potential of this novel waterfowl parvovirus, cherry valley ducklings (n = 20) were experimentally infected with N-GPV strain SDLC01. An equal number of ducklings served as uninfected controls. The appearance of clinical signs, histopathological changes, viral shedding, and seroconversion was monitored for 20 days post-infection. Infection status of all ducks was monitored using indirect ELISA, virus neutralization test, nested PCR, clinical indicators, and microscopic examination. Three ducks developed the typical clinical, gross, and histological changes of BADS. By study day 6, the infected ducks had seroconverted to N-GPV. The antibodies raised were neutralizing against the SDLC01 strain in vitro. Here we successfully developed an experimental infection model for studying the pathogenicity and role of N-GPV in BADS.
Journal: Veterinary Microbiology - Volume 183, 1 February 2016, Pages 16-20