کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5799927 1555351 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Pathogenic characteristics of Marek's disease virus field strains prevalent in China and the effectiveness of existing vaccines against them
ترجمه فارسی عنوان
ویژگیهای بیماریزای گونه های سرطانی بیماری ویروس مارک شایع در چین و اثربخی واکسن های موجود در برابر آنها است
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی


- MDV strains were isolated from the feather pulps of Chinese vaccinated flocks.
- The CVI988 vaccine provides good protection against the LCC and LLY strains.
- The CVI988 vaccine cannot effectively protect against the LTS strain.

The virulence of Marek's disease virus (MDV) is continuously evolving, and more virulent MDV pathotypes are emerging, thereby reducing the effectiveness of the existing vaccines. In this study, feather pulps were collected from diseased chickens in commercial chicken flocks in China that presented significant MD visceral tumors in 2011 and were inoculated into a monolayer of duck embryo fibroblasts (DEFs). Three field isolates of MDV were obtained by plaque cloning and identified as MDV via PCR and designated strains LCC, LLY, and LTS. Unvaccinated and CVI988 vaccine-vaccinated specific pathogen-free chickens were challenged at 7 days post vaccination (dpv) with 1000 plaque forming units of each of the respective MDV isolates. These strains induced gross MD lesions in all (100%) of the unvaccinated chickens, and the mortality rates of the unvaccinated chickens were 42.9%, 46.7%, and 23.1% by 60 days post challenge (dpc), respectively. The CVI988 vaccine induced protective indices (PIs) of 85.7, 92.3, and 66.7, respectively. These results showed that the pathogenic characteristics of the Chinese isolates were diverse and that vaccine CVI988 provided different levels of protection against them. These data indicated that the existence of variant MDV strains was a possible reason of immunity failure in China.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Microbiology - Volume 177, Issues 1–2, 15 May 2015, Pages 62-68
نویسندگان
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