کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5800005 1555352 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Carbopol improves the early cellular immune responses induced by the modified-life vaccine Ingelvac PRRS® MLV
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Carbopol improves the early cellular immune responses induced by the modified-life vaccine Ingelvac PRRS® MLV
چکیده انگلیسی


- Adjuvants impact modified life-attenuated vaccines.
- Carbopol adjuvant improves Ingelvac PRRSV MLV in piglets.
- We quantified IFNg production and memory CD4T cell differentiation.
- Carbopol induces early IFNg and promotes differentiation to effector phenotypes.

SummaryAdjuvants enhance both the magnitude and duration of immune responses, therefore representing a central component of vaccines. The nature of the adjuvant can determine the particular type of immune response, which may be skewed toward cytotoxic T cell (CTL) responses, antibody responses, or particular classes of T helper (Th) responses and antibody isotypes. Traditionally, adjuvants have been added to intrinsically poor immunogenic vaccines, such as those using whole killed organisms or subunit vaccines. Here, we have compared cellular immune responses induced by the immunogenic modified life-attenuated vaccine Ingelvac PRRS® MLV when administered alone or in combination with carbopol, a widely used adjuvant in veterinary medicine. Using functional readouts (IFN-γ ELISpot and cell proliferation) and analyzing phenotypical hallmarks of CD4T cell differentiation, we show that carbopol improves cellular immunity by inducing early IFN-γ-producing cells and by preferentially driving T cell differentiation to effector phenotypes. Our data suggest that adjuvants may enhance and modulate life-attenuated - not only subunit/inactivated - vaccines.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Microbiology - Volume 176, Issues 3–4, 17 April 2015, Pages 352-357
نویسندگان
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