کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5800328 1555350 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Pseudorabies virus US3 leads to filamentous actin disassembly and contributes to viral genome delivery to the nucleus
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Pseudorabies virus US3 leads to filamentous actin disassembly and contributes to viral genome delivery to the nucleus
چکیده انگلیسی


- Pseudorabies virus (PRV) triggers filamentous actin (F-actin) disassembly.
- The kinase activity of US3 is required for F-actin disassembly.
- US3 of PRV plays a role in viral genome delivery to the nucleus.
- Disassembly of filamentous actin enhances viral genome delivery to the nucleus.

The conserved alphaherpesvirus US3 tegument protein induces rearrangements of the actin cytoskeleton, consisting of protrusion formation and stress fiber breakdown. Although US3 does not affect levels of total actin protein, it remains unclear whether US3 modulates the total levels of filamentous (F) actin. In this report, we show that the pseudorabies virus (PRV) US3 protein, via its kinase activity, leads to disassembly of F-actin in porcine ST cells. F-actin disassembly has been reported before to contribute to host cell entry of HIV. In line with this, in the current study, we report that US3 has a previously uncharacterized role in viral genome delivery to the nucleus, since quantitative polymerase chain reaction (qPCR) assays on nuclear fractions demonstrated a reduced nuclear delivery of US3null PRV compared to wild type PRV genomes. Treatment of cells with the actin depolymerizing drug cytochalasin D enhanced virus genome delivery to the nucleus, particularly of US3null PRV, supporting a role for F-actin disassembly during certain aspects of viral entry. In conclusion, the US3 kinase of PRV leads to F-actin depolymerization, and US3 and F-actin disassembly contribute to viral genome delivery to the nucleus.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Microbiology - Volume 177, Issues 3–4, 12 June 2015, Pages 379-385
نویسندگان
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