کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5800442 1555355 2014 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Linear epitope recognition antibodies strongly respond to the C-terminal domain of HP-PRRSV GP5
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Linear epitope recognition antibodies strongly respond to the C-terminal domain of HP-PRRSV GP5
چکیده انگلیسی


- Peptides scan was used to analysis HP-PRRSV GP5 antigen characters.
- We created a HP-PRRSV GP5 antigen reaction panorama.
- HP-PRRSV specific antibodies strongly respond to the C-terminal domain of GP5.
- Seventeen epitope candidates were arisen in GP5 in this research.

A total of 155 peptides derived from the highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) glycoprotein 5 (GP5) were printed on a chip to reveal the antigen reaction characteristics of the protein. The reactions of these peptides to HP-PRRSV-specific pig serum were scanned and quantified using fluorescence intensity via the PepSlide® Analyzer software. The intensity plots showed different reactions in the different sectors of GP5. The highest reaction intensity value reached 3894.5, with a peptide sequence of IVEKGGKVEVEGHLI. Seventeen peptides that showed relatively high reaction levels with HP-PRRSV-specific pig serum were selected as epitope candidates. Furthermore, the antigenic character was predicted using a software and was compared with the peptide scan results. In contrast to the software prediction, the HP-PRRSV-specific antibodies strongly responded to the C-terminal domain of GP5. The acquired data may be useful for understanding the antigenic characteristics of HP-PRRSV GP5.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Microbiology - Volume 174, Issues 3–4, 5 December 2014, Pages 565-569
نویسندگان
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