کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5800468 | 1555358 | 2014 | 10 صفحه PDF | دانلود رایگان |
- We analysed AlHV-1 virus proteins by proteomics and western blotting.
- This identified eight virus proteins as candidate antigens.
- Capsid and tegument candidates from OvHV-2 and AlHV-1 were expressed in bacteria.
- Blotting showed that only ORF65 and ORF17.5 proteins were antigenic.
- ORF65 was strongly detected by both vaccinated and infected cattle serum.
Malignant catarrhal fever (MCF) is a fatal disease of cattle and other ungulates caused by certain gamma-herpesviruses including alcelaphine herpesvirus-1 (AlHV-1) and ovine herpesvirus-2 (OvHV-2). An attenuated virus vaccine based on AlHV-1 has been shown to induce virus-neutralising antibodies in plasma and nasal secretions of protected cattle but the targets of virus-specific antibodies are unknown. Proteomic analysis and western blotting of virus extracts allowed the identification of eight candidate AlHV-1 virion antigens. Recombinant expression of selected candidates and their OvHV-2 orthologues confirmed that two polypeptides, the products of the ORF17.5 and ORF65 genes, were antigens recognised by antibodies from natural MCF cases or from AlHV-1 vaccinated cattle. These proteins have potential as diagnostic and/or vaccine antigens.
Journal: Veterinary Microbiology - Volume 173, Issues 1â2, 17 September 2014, Pages 17-26